Attempts were made to grow 4 isolates of Cowdria ruminantium in cell lines. Three of these isolates, viz.
Ball 3, Welgevonden and Kwanyanga, could be cultivated in a calf endothelial cell line, but experiments with the Kumm isolate have so far failed.
The successful in vitro cultivation of 2 isolates (Welgevonden and Kwanyanga), which are also pathogenic
for mice, has great potential for future studies and these aspects are discussed in this review.