A new species of relatively poorly known genus Zambedania Mahunka, 1972 was
found on the baboon spider, Harpactirella overdijki Gallon, 2010 (Araneae: Theraphosidae) in South Africa.
Besides the abundantly available phoretic females, several males and one larva of this species in the spiders’
nests were also collected. Zambedania sekhukhunensis n. sp. is described and illustrated based on the phoretic
females, males and larva. Improved diagnosis of the genus and a new key to species are also supplied. The
descriptions and illustrations of the male and larva of this species represent the first ones of these stages in the
genus Zambedania. Due to their discovery the generic diagnosis has been significantly improved.