The aim of this study was to determine whether storage
time had a significant effect on the platelet concentration of
platelet-rich fibrin (PRF). Three blood samples were drawn
from each participant into a sterile blood sampling tube.
Two of the blood samples were centrifuged to form PRF.
The third non-centrifuged sample was used to measure
the baseline blood platelet concentration. After PRF had
formed, it was removed from the respective test tubes at
different time intervals i.e. immediately after centrifugation
(Group A) and after 60 min of storage time in the blood
collecting tube (Group B). The residual blood from each
group was tested for platelet concentration and compared
with the baseline reading (as an indirect measure of the
platelet concentrate of PRF). The PRF produced in Group
A (PRF A) had a mean platelet concentration of 274
+ - 57.8 X 109/L, whereas the PRF of Group B (PRF B) was
278 + - 58.2 X 109/L. A statistically significant difference was
seen between the groups (p < 0.001).