Rev 1 vaccination of sheep induced complement fixation titres to both Brucella abortus and Brucella
ovis antigens, the complement fixing antibody titres were, however, higher with the B. abortus antigen.
These titres generally fell to negative levels (below 1/20) within 5 to 6 months of vaccination. In goats
Rev 1 vaccination induced complement fixation titres against B. abortus antigen which fell to negative levels within 5 to 6 months. Agglutination at 37°C or 56°C, agglutination in 5% NaCl, and Coombs tests are less useful in vaccinated animals as titres remain at positive levels for much longer periods after vaccination.