Purpose This study reports the incorporation of the antibiotics
rifampin, doxycycline and clarithromycin in poly(styrene-co-methyl
methacrylate films and their effect on biofilm prevention.
Background Invasive procedures in patients such as surgical
device, or intravenous or urinary catheter implantation, often
results in complicated hospital-acquired nosocomial infections.
Biofilm formation is essential to establish these infections on these
devices and novel antibiotic delivery approaches are needed for
more effective management.
Methods The films were evaluated in vitro for drug release and
for their ability to prevent biofilm formation by methicillin susceptible
and methicillin resistant Staphylococcus aureus. Surface tension
components, obtained from contact angle measurements,
and the morphology of the films evaluated by scanning electron
microscopy were also investigated.
Results In this study, antibiotic-loaded methacrylic copolymer
films that effectively released rifampin, clarithromycin and doxycycline
for up to 21 days prevented biofilm formation when tested in
an in vitro bioreactor model. These drug loaded copolymer films
provided the advantage by coating materials with a novel surface
that was unsuitable for resettling of biofilms once the antibiotic was
dissolved from the polymer surface. A combination of rifampin
and clarithromycin released from the polymer film provided
>99.9% kill of an MRSA inoculate for up to 72 h.
Conclusion Results showed that combining multiple drugs in
copolymer films with unique surface properties, initial hydrophilicity
and increase in roughness, can be an effective way to prevent