An accurate, sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma

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Peer-Reviewed Research
  • SDG 12
  • SDG 2
  • Abstract:

    A sensitive and reproducible method permitting the estimation of 0,030-3,000 mM of free fatty acids (FFA) in plasma with a standard error of 0,006 5 was developed. The method is based on the extraction of phosphate-buffered plasma with a chloroform-heptane-methanol mixture, formation of the cobalt complex and on the subsequent determination of the metal with 1-nitroso-2 naphthol. The method was used to obtain normal values (mean 0,547 mM range 0,147-1,197 mM) for Merino wethers fed lucerne hay.