Structural characterization and directed evolution of a novel acetyl xylan esterase reveals thermostability determinants of the carbohydrate esterase 7 family
20 Apr 2018Please read abstract in the article.
| Authors: | Adesioye, Fiyinfoluwa Adenike, Makhalanyane, Thulani P., Vikram, Surendra, Sewell, Bryan T., Schubert, Wolf-Dieter, Cowan, Don A. |
| Institution: | University of Pretoria |
| Keywords: | X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability, X-ray crystallography, Structural characterization, Single-point mutation, Salt concentration, Metagenomics, Directed evolution, Catalytic efficiencies, Biosynthesis, Carbohydrates, Catalyst activity, Cloning, Crystal structure, Efficiency, Enzymes, Esters, Genes, Sodium compounds, Thermodynamic stability, Acetyl xylan esterase (AcXE), Carbohydrate esterase 7 (CE7), Sequence-based metagenomics, Thermal stability |