Increasing the scale of peroxidase production by Streptomyces sp. strain BSII#1

19 Jul 2016

Aims To optimize peroxidase production by Streptomyces sp. strain BSII#1, up to 3 l culture volumes. Methods and Results Peroxidase production by Streptomyces sp. strain BSII#1 was optimized in terms of production temperature and pH and the use of lignin-based model chemical inducers. The highest peroxidase activity (1?30 ? 0?04 U ml?1) in 10 ml culture volume was achieved in a complex production medium (pH 8?0) at 37?C in the presence of 0?1 mmol l?1 veratryl alcohol, which was greater than those reported previously. Scale-up to 100 and 400 ml culture volumes resulted in decreased peroxidase production (0?53 ? 0?10 and 0?26 ? 0?08 U ml?1, respectively). However, increased aeration improved peroxidase production with the highest production achieved using an airlift bioreactor (4?76 ? 0?46 U ml?1 in 3 l culture volume). Conclusions Veratryl alcohol (0?1 mmol l?1) is an effective inducer of peroxidase production by Streptomyces sp. strain BSII#1. However, improved aeration increased peroxidase production in larger volumes without the use of an inducer, surpassing induced yields in an optimized small-scale process. Significance and Impact of the Study Only a limited number of reports in literature have focused on the up-scaling of bacterial peroxidase production. There remains opportunity for feasible large-scale production of bacterial peroxidases with potentially novel biocatalytic properties.